Immunohistochemical demonstration of interleukin-1 β induced changes in acute-phase proteins and albumin in rat liver
Identifieur interne : 000E87 ( Main/Exploration ); précédent : 000E86; suivant : 000E88Immunohistochemical demonstration of interleukin-1 β induced changes in acute-phase proteins and albumin in rat liver
Auteurs : Peter E. Ballmer [Suisse] ; Reinhard Imoberdorf [Suisse] ; Jakob Zb Ren [Suisse]Source :
- Acta Histochemica [ 0065-1281 ] ; 1995.
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Abstract
Interleukin-1β is a potent mediator of the acute-phase response. However, the effects of interleukin-1β administration on the topic in vivo production of acute-phase proteins and albumin are so far not well understood. Overnight fasted rats were subcutaneously injected with 0.2 mL 0.9% NaCl (control group) or 6.25 μg recombinant human interleukin-1β, rectal temperature was measured at intervals up to 48 h. Livers were perfused-fixed in vivo prior to injection (base-line), at 9, 24, 48 h following the interleukin-1β injection. Fibrinogen, orosomucoid (α1-acid glycoprotein) and albumin were immunostained using a streptavidin-biotin-immunoperoxidase technique. Rectal temperature peaked 5 h after the single interleukin-1β injection, fell gradually to base-line values by 24 h. Prior to injection only a few hepatocytes, randomly scattered throughout the liver lobule, stained positive for fibrinogen and orosomucoid. In contrast, all hepatocytes stained uniformly positive for fibrinogen and orosomucoid 9 h after interleukin-1β injection, whereas at 24 h a predominant centrilobular staining pattern occurred. Due to fasting, albumin positive hepatocytes were already reduced at base-line in both groups. Interleukin-1β induced a further significant loss of albumin positive cells in the periportal zone (35 ± 21%): at 9 h when compared with controls (58 ± 11%, p = 0.037). In conclusion, subcutaneous interleukin-1β (probably by stimulation of interleukin-6) strongly induces fibrinogen and orosomucoid expression in rat liver, suppresses immunohistochemically stainable albumin in a heterogenous way, mainly in the periportal zone.
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DOI: 10.1016/S0065-1281(11)80190-X
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However, the effects of interleukin-1β administration on the topic in vivo production of acute-phase proteins and albumin are so far not well understood. Overnight fasted rats were subcutaneously injected with 0.2 mL 0.9% NaCl (control group) or 6.25 μg recombinant human interleukin-1β, rectal temperature was measured at intervals up to 48 h. Livers were perfused-fixed in vivo prior to injection (base-line), at 9, 24, 48 h following the interleukin-1β injection. Fibrinogen, orosomucoid (α1-acid glycoprotein) and albumin were immunostained using a streptavidin-biotin-immunoperoxidase technique. Rectal temperature peaked 5 h after the single interleukin-1β injection, fell gradually to base-line values by 24 h. Prior to injection only a few hepatocytes, randomly scattered throughout the liver lobule, stained positive for fibrinogen and orosomucoid. In contrast, all hepatocytes stained uniformly positive for fibrinogen and orosomucoid 9 h after interleukin-1β injection, whereas at 24 h a predominant centrilobular staining pattern occurred. Due to fasting, albumin positive hepatocytes were already reduced at base-line in both groups. Interleukin-1β induced a further significant loss of albumin positive cells in the periportal zone (35 ± 21%): at 9 h when compared with controls (58 ± 11%, p = 0.037). In conclusion, subcutaneous interleukin-1β (probably by stimulation of interleukin-6) strongly induces fibrinogen and orosomucoid expression in rat liver, suppresses immunohistochemically stainable albumin in a heterogenous way, mainly in the periportal zone.</div></front></TEI><affiliations><list><country><li>Suisse</li></country></list><tree><country name="Suisse"><noRegion><name sortKey="Ballmer, Peter E" sort="Ballmer, Peter E" uniqKey="Ballmer P" first="Peter E." last="Ballmer">Peter E. Ballmer</name></noRegion><name sortKey="Imoberdorf, Reinhard" sort="Imoberdorf, Reinhard" uniqKey="Imoberdorf R" first="Reinhard" last="Imoberdorf">Reinhard Imoberdorf</name><name sortKey="Zb Ren, Jakob" sort="Zb Ren, Jakob" uniqKey="Zb Ren J" first="Jakob" last="Zb Ren">Jakob Zb Ren</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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